› 2qr0

X-ray diffraction
3.5Å resolution

Structure of VEGF complexed to a Fab containing TYR and SER in the CDRs

Released:
DOI: 10.2210/pdb2qr0/pdb
PDB entry 2qr0 coloured by chain, front view.
PDB entry 2qr0 coloured by chain, side view.
PDB entry 2qr0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Fellouse FA, Esaki K, Birtalan S, Raptis D, Cancasci VJ, Koide A, Jhurani P, Vasser M, Wiesmann C, Kossiakoff AA, Koide S, Sidhu SS
J Mol Biol 373 924-40 (2007)
PMID: 17825836

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210647 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab-Fragment Light Chain Chains: A, E, G, K, M, Q, S, W
Molecule details ›
Chains: A, E, G, K, M, Q, S, W
Length: 213 amino acids
Theoretical weight: 23.5 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Fab-Fragment Heavy Chain Chains: B, F, H, L, N, R, T, X
Molecule details ›
Chains: B, F, H, L, N, R, T, X
Length: 221 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
N-VEGF Chains: C, D, I, J, O, P, U, V
Molecule details ›
Chains: C, D, I, J, O, P, U, V
Length: 97 amino acids
Theoretical weight: 11.37 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P15692 (Residues: 219-315; Coverage: 25%)
Gene names: VEGF, VEGFA
Structure domains: Cystine-knot cytokines

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 154.453Å b: 90.218Å c: 205.558Å
α: 90° β: 90.85° γ: 90°
R-values:
R R work R free
0.292 0.291 0.312

Quick links

Citations

36 review citations

Molecular engineering and design of therapeutic antibodies.
Presta LG. (2008)
35 more

7 mentions without citation

The importance of being tyrosine: lessons in molecular recognition from minimalist synthetic binding proteins.
Koide et al. (2009)
6 more