› 2qra

X-ray diffraction
2.5Å resolution

Crystal structure of XIAP BIR1 domain (P21 form)

Released:
DOI: 10.2210/pdb2qra/pdb
PDB entry 2qra coloured by chain, front view.
PDB entry 2qra coloured by chain, side view.
PDB entry 2qra coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the BIR1 domain of XIAP in two crystal forms.
Lin SC, Huang Y, Lo YC, Lu M, Wu H
J Mol Biol 372 847-854 (2007)
PMID: 17698078

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase XIAP Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 111 amino acids
Theoretical weight: 12.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P98170 (Residues: 10-99; Coverage: 18%)
Gene names: API3, BIRC4, IAP3, XIAP
Sequence domains: Inhibitor of Apoptosis domain
Structure domains: Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand EOH 6 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 36.563Å b: 72.983Å c: 68.904Å
α: 90° β: 95.69° γ: 90°
R-values:
R R work R free
0.196 0.196 0.253
Expression system: Escherichia coli K-12

Quick links

Citations

9 review citations

IAPs: from caspase inhibitors to modulators of NF-kappaB, inflammation and cancer.
Gyrd-Hansen et al. (2010)
8 more

3 mentions without citation

Molecular modeling in the age of clinical genomics, the enterprise of the next generation.
Prokop et al. (2017)
2 more