› 2qsz

X-ray diffraction
1.9Å resolution

Human nicotinamide riboside kinase 1 in complex with nicotinamide mononucleotide

Released:
DOI: 10.2210/pdb2qsz/pdb
PDB entry 2qsz coloured by chain, front view.
PDB entry 2qsz coloured by chain, side view.
PDB entry 2qsz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nicotinamide riboside kinase structures reveal new pathways to NAD+.
Tempel W, Rabeh WM, Bogan KL, Belenky P, Wojcik M, Seidle HF, Nedyalkova L, Yang T, Sauve AA, Park HW, Brenner C
PLoS Biol 5 e263 (2007)
PMID: 17914902

Function and Biology Details

Reactions catalysed: 
ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192664 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide riboside kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 24.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NWW6 (Residues: 2-189; Coverage: 95%)
Gene names: C9orf95, NMRK1, NRK1
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand PO4 1 x PO4
Ligand CL 1 x CL
Ligand NMN 1 x NMN
Ligand UNX 7 x UNX
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C2221
Unit cell:
a: 55.921Å b: 142.342Å c: 62.434Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.255
Expression system: Escherichia coli

Quick links

Citations

38 review citations

Nicotinic acid, nicotinamide, and nicotinamide riboside: a molecular evaluation of NAD+ precursor vitamins in human nutrition.
Bogan et al. (2008)
37 more

1 mention without citation

Nicotinamide riboside kinase structures reveal new pathways to NAD+.
Tempel et al. (2007)