› 2r05

X-ray diffraction
2.55Å resolution

Crystal Structure of ALIX/AIP1 in complex with the HIV-1 YPLASL Late Domain

Released:
DOI: 10.2210/pdb2r05/pdb
PDB entry 2r05 coloured by chain, front view.
PDB entry 2r05 coloured by chain, side view.
PDB entry 2r05 coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV.
Zhai Q, Fisher RD, Chung HY, Myszka DG, Sundquist WI, Hill CP
Nat Struct Mol Biol 15 43-9 (2008)
PMID: 18066081

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153077 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Programmed cell death 6-interacting protein Chain: A
Molecule details ›
Chain: A
Length: 697 amino acids
Theoretical weight: 78.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WUM4 (Residues: 2-698; Coverage: 80%)
Gene names: AIP1, ALIX, KIAA1375, PDCD6IP
Sequence domains:
Structure domains:
p6-gag Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Human immunodeficiency virus type 1 (isolate YU2)
Expression system: Not provided
UniProt:
  • Canonical: P35962 (Residues: 482-492; Coverage: 2%)
Gene name: gag

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 146.09Å b: 99.44Å c: 73.543Å
α: 90° β: 107.29° γ: 90°
R-values:
R R work R free
0.228 0.225 0.28
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

30 review citations

HIV-1 assembly, budding, and maturation.
Sundquist et al. (2012)
29 more

1 mention without citation

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)