› 2r0k

X-ray diffraction
3.51Å resolution

Protease domain of HGFA with inhibitor Fab58

Released:
DOI: 10.2210/pdb2r0k/pdb
PDB entry 2r0k coloured by chain, front view.
PDB entry 2r0k coloured by chain, side view.
PDB entry 2r0k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into distinct mechanisms of protease inhibition by antibodies.
Wu Y, Eigenbrot C, Liang WC, Stawicki S, Shia S, Fan B, Ganesan R, Lipari MT, Kirchhofer D
Proc Natl Acad Sci U S A 104 19784-9 (2007)
PMID: 18077410

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211912 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hepatocyte growth factor activator long chain Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 30.89 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q04756 (Residues: 373-655; Coverage: 46%)
Gene name: HGFAC
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
antibody light chain of Fab58 Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
antibody heavy chain of Fab58, Fab portion only Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 23.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 188.658Å b: 75.61Å c: 69.139Å
α: 90° β: 92.67° γ: 90°
R-values:
R R work R free
0.254 0.251 0.312
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

10 review citations

Targeting MET in cancer: rationale and progress.
Gherardi et al. (2012)
9 more

6 mentions without citation

Structural insight into distinct mechanisms of protease inhibition by antibodies.
Wu et al. (2007)
5 more