› 2r0n

X-ray diffraction
2.3Å resolution

The effect of a Glu370Asp mutation in Glutaryl-CoA Dehydrogenase on Proton Transfer to the Dienolate Intermediate

Released:
DOI: 10.2210/pdb2r0n/pdb
PDB entry 2r0n coloured by chain, front view.
PDB entry 2r0n coloured by chain, side view.
PDB entry 2r0n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The effect of a Glu370Asp mutation in glutaryl-CoA dehydrogenase on proton transfer to the dienolate intermediate.
Rao KS, Fu Z, Albro M, Narayanan B, Baddam S, Lee HJ, Kim JJ, Frerman FE
Biochemistry 46 14468-77 (2007)
PMID: 18020372

Function and Biology Details

Reaction catalysed: 
(1a) glutaryl-CoA + electron-transfer flavoprotein = (E)-glutaconyl-CoA + reduced electron-transfer flavoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-187819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaryl-CoA dehydrogenase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 43.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92947 (Residues: 45-438; Coverage: 90%)
Gene name: GCDH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand TGC 1 x TGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6422
Unit cell:
a: 114.65Å b: 114.65Å c: 126.78Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.284
Expression system: Escherichia coli BL21

Quick links

Citations

5 citation in other articles

Decarboxylating and nondecarboxylating glutaryl-coenzyme A dehydrogenases in the aromatic metabolism of obligately anaerobic bacteria.
Wischgoll et al. (2009)
4 more

2 mentions without citation

Highly Accessible Computational Prediction and In Vivo/In Vitro Experimental Validation: Novel Synthetic Phenyl Ketone Derivatives as Promising Agents against NAFLD via Modulating Oxidoreductase Activity.
Qiao et al. (2023)
1 more