› 2r56

X-ray diffraction
2.8Å resolution

Crystal Structure of a Recombinant IgE Fab Fragment in Complex with Bovine Beta-Lactoglobulin Allergen

Released:
DOI: 10.2210/pdb2r56/pdb
PDB entry 2r56 coloured by chain, front view.
PDB entry 2r56 coloured by chain, side view.
PDB entry 2r56 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen.
Niemi M, Jylhä S, Laukkanen ML, Söderlund H, Mäkinen-Kiljunen S, Kallio JM, Hakulinen N, Haahtela T, Takkinen K, Rouvinen J
Structure 15 1413-21 (2007)
PMID: 17997967

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208785 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-lactoglobulin Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.3 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02754 (Residues: 17-178; Coverage: 100%)
Gene name: LGB
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin
IgE Fab Fragment, light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 211 amino acids
Theoretical weight: 23.12 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
IgE Fab Fragment, heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 221 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand LMT 2 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 67.04Å b: 100.64Å c: 168.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.291
Expression system: Escherichia coli

Quick links

Citations

17 review citations

IgE in allergy and asthma today.
Gould et al. (2008)
16 more

23 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
22 more