› 2rba

X-ray diffraction
2.79Å resolution

Structure of Human Thymine DNA Glycosylase Bound to Abasic and Undamaged DNA

Released:
DOI: 10.2210/pdb2rba/pdb
PDB entry 2rba coloured by chain, front view.
PDB entry 2rba coloured by chain, side view.
PDB entry 2rba coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human thymine DNA glycosylase bound to DNA elucidates sequence-specific mismatch recognition.
Maiti A, Morgan MT, Pozharski E, Drohat AC
Proc Natl Acad Sci U S A 105 8890-5 (2008)
PMID: 18587051

Function and Biology Details

Reaction catalysed: 
Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115831 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
G/T mismatch-specific thymine DNA glycosylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 23.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13569 (Residues: 111-308; Coverage: 48%)
Gene name: TDG
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain
DNA (5'-D(*DCP*DAP*DGP*DCP*DTP*DCP*DTP*DGP*DTP*DAP*DCP*DGP*DTP*DGP*DAP*DGP*DCP*DAP*DGP*DTP*DGP*DGP*DA)-3') Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7.12 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*DCP*DCP*DAP*DCP*DTP*DGP*DCP*DTP*DCP*DAP*(3DR)P*DGP*DTP*DAP*DCP*DAP*DGP*DAP*DGP*DCP*DTP*DGP*DT)-3') Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 6.89 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P65
Unit cell:
a: 162.133Å b: 162.133Å c: 56.142Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.23 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

DNA glycosylases: in DNA repair and beyond.
Jacobs et al. (2012)
15 more

21 mentions without citation

Detection of damaged DNA bases by DNA glycosylase enzymes.
Friedman et al. (2010)
20 more