› 2rd8

X-ray diffraction
2.5Å resolution

Human Thymidylate Synthase Stabilized in Active Conformation by R163K Mutation: Asymmetry and Reactivity of Cys195

Released:
DOI: 10.2210/pdb2rd8/pdb
PDB entry 2rd8 coloured by chain, front view.
PDB entry 2rd8 coloured by chain, side view.
PDB entry 2rd8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The R163K mutant of human thymidylate synthase is stabilized in an active conformation: structural asymmetry and reactivity of cysteine 195.
Gibson LM, Lovelace LL, Lebioda L
Biochemistry 47 4636-43 (2008)
PMID: 18370400

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138194 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thymidylate synthase Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 35.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04818 (Residues: 1-313; Coverage: 100%)
Gene names: OK/SW-cl.29, TS, TYMS
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain
Thymidylate synthase Chain: B
Molecule details ›
Chain: B
Length: 313 amino acids
Theoretical weight: 35.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04818 (Residues: 1-313; Coverage: 100%)
Gene names: OK/SW-cl.29, TS, TYMS
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

2 bound ligands:
Ligand BME 1 x BME
Ligand PO4 2 x PO4
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P3221
Unit cell:
a: 120.741Å b: 120.741Å c: 129.771Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.228 0.271
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Protein-protein interface-binding peptides inhibit the cancer therapy target human thymidylate synthase.
Cardinale et al. (2011)
9 more

6 mentions without citation

Analysis of mRNA recognition by human thymidylate synthase.
Brunn et al. (2014)
5 more