› 2rgz

X-ray diffraction
2.61Å resolution

Ensemble refinement of the protein crystal structure of human heme oxygenase-2 C127A (HO-2) with bound heme

Released:
DOI: 10.2210/pdb2rgz/pdb
PDB entry 2rgz coloured by chain, ensemble of 16 models, front view.
PDB entry 2rgz coloured by chain, ensemble of 16 models, side view.
PDB entry 2rgz coloured by chain, ensemble of 16 models, top view.
Source organism: Homo sapiens
Primary publication:
Comparison of apo- and heme-bound crystal structures of a truncated human heme oxygenase-2.
Bianchetti CM, Yi L, Ragsdale SW, Phillips GN
J Biol Chem 282 37624-31 (2007)
PMID: 17965015

Function and Biology Details

Reaction catalysed: 
Protoheme + 3 [reduced NADPH--hemoprotein reductase] + 3 O(2) = biliverdin + Fe(2+) + CO + 3 [oxidized NADPH--hemoprotein reductase] + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme oxygenase 2 soluble form Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 30.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30519 (Residues: 1-264; Coverage: 84%)
Gene names: HMOX2, HO2
Sequence domains: Heme oxygenase
Structure domains: Heme oxygenase-like

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 74.977Å b: 85.094Å c: 97.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
10 more

6 mentions without citation

Comparison of apo- and heme-bound crystal structures of a truncated human heme oxygenase-2.
Bianchetti et al. (2007)
5 more