› 2rh1

X-ray diffraction
2.4Å resolution

High resolution crystal structure of human B2-adrenergic G protein-coupled receptor.

Released:
DOI: 10.2210/pdb2rh1/pdb
PDB entry 2rh1 coloured by chain, front view.
PDB entry 2rh1 coloured by chain, side view.
PDB entry 2rh1 coloured by chain, top view.
Source organisms:
Primary publication:
High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor.
Cherezov V, Rosenbaum DM, Hanson MA, Rasmussen SG, Thian FS, Kobilka TS, Choi HJ, Kuhn P, Weis WI, Kobilka BK, Stevens RC
Science 318 1258-65 (2007)
PMID: 17962520

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-2 adrenergic receptor; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 56.6 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07550 (Residues: 1-230, 264-365; Coverage: 80%)
  • Canonical: P00720 (Residues: 2-162; Coverage: 98%)
Gene names: ADRB2, ADRB2R, B2AR, E
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
7 bound ligands:
Ligand SO4 6 x SO4
Ligand CAU 1 x CAU
Ligand BU1 2 x BU1
Ligand ACM 1 x ACM
Ligand CLR 3 x CLR
Ligand PLM 1 x PLM
Ligand 12P 1 x 12P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 106.318Å b: 169.24Å c: 40.154Å
α: 90° β: 105.62° γ: 90°
R-values:
R R work R free
0.198 0.196 0.232
Expression system: Spodoptera frugiperda

Quick links

Citations

374 review citations

Lipid rafts as a membrane-organizing principle.
Lingwood et al. (2010)
373 more

674 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
673 more