› 2rjf

X-ray diffraction
2.05Å resolution

Crystal structure of L3MBTL1 in complex with H4K20Me2 (residues 12-30), orthorhombic form I

Released:
DOI: 10.2210/pdb2rjf/pdb
PDB entry 2rjf coloured by chain, front view.
PDB entry 2rjf coloured by chain, side view.
PDB entry 2rjf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
L3MBTL1 recognition of mono- and dimethylated histones.
Min J, Allali-Hassani A, Nady N, Qi C, Ouyang H, Liu Y, MacKenzie F, Vedadi M, Arrowsmith CH
Nat Struct Mol Biol 14 1229-30 (2007)
PMID: 18026117

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
hetero tetramer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-163225 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lethal(3)malignant brain tumor-like protein 1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 331 amino acids
Theoretical weight: 37.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y468 (Residues: 268-598; Coverage: 39%)
Gene names: KIAA0681, L3MBT, L3MBTL, L3MBTL1
Sequence domains: mbt repeat
Structure domains: SH3 type barrels.
Histone H4 Chains: B, D
Molecule details ›
Chains: B, D
Length: 20 amino acids
Theoretical weight: 2.34 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 2 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ DW
Spacegroup: P212121
Unit cell:
a: 90.458Å b: 117.809Å c: 132.316Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.225
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

56 review citations

Double-strand break repair: 53BP1 comes into focus.
Panier et al. (2014)
55 more

6 mentions without citation

Chemical modulators for epigenome reader domains as emerging epigenetic therapies for cancer and inflammation.
Zaware et al. (2017)
5 more