› 2rnw

Solution NMR

The Structural Basis for Site-Specific Lysine-Acetylated Histone Recognition by the Bromodomains of the Human Transcriptional Co-Activators PCAf and CBP

Released:
DOI: 10.2210/pdb2rnw/pdb
PDB entry 2rnw coloured by chain, ensemble of 20 models, front view.
PDB entry 2rnw coloured by chain, ensemble of 20 models, side view.
PDB entry 2rnw coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Structural basis of site-specific histone recognition by the bromodomains of human coactivators PCAF and CBP/p300.
Zeng L, Zhang Q, Gerona-Navarro G, Moshkina N, Zhou MM
Structure 16 643-52 (2008)
PMID: 18400184

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158416 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone acetyltransferase KAT2B Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 14.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92831 (Residues: 719-832; Coverage: 14%)
Gene names: KAT2B, PCAF
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Histone H3 Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.61 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P61830 (Residues: 2-16; Coverage: 11%)
Gene names: HHT1, HHT2, N2749, SIN2, YBR010W, YBR0201, YNL031C

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Protein lysine acetylation by p300/CBP.
Dancy et al. (2015)
29 more

3 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
2 more