› 2rny

Solution NMR

Complex Structures of CBP Bromodomain with H4 ack20 Peptide

Released:
DOI: 10.2210/pdb2rny/pdb
PDB entry 2rny coloured by chain, ensemble of 20 models, front view.
PDB entry 2rny coloured by chain, ensemble of 20 models, side view.
PDB entry 2rny coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of site-specific histone recognition by the bromodomains of human coactivators PCAF and CBP/p300.
Zeng L, Zhang Q, Gerona-Navarro G, Moshkina N, Zhou MM
Structure 16 643-52 (2008)
PMID: 18400184

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158685 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CREB-binding protein Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 14.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92793 (Residues: 1081-1197; Coverage: 5%)
Gene names: CBP, CREBBP
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Histone H4 Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62805 (Residues: 14-28; Coverage: 15%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli BL21

Quick links

Citations

28 review citations

Bromodomains as therapeutic targets.
Muller et al. (2011)
27 more

7 mentions without citation

Protein lysine acetylation by p300/CBP.
Dancy et al. (2015)
6 more