› 2rsn

Solution NMR

Solution structure of the chromodomain of Chp1 in complex with H3K9me3 peptide

Released:
DOI: 10.2210/pdb2rsn/pdb
PDB entry 2rsn coloured by chain, ensemble of 20 models, front view.
PDB entry 2rsn coloured by chain, ensemble of 20 models, side view.
PDB entry 2rsn coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Intrinsic nucleic acid-binding activity of Chp1 chromodomain is required for heterochromatic gene silencing.
Ishida M, Shimojo H, Hayashi A, Kawaguchi R, Ohtani Y, Uegaki K, Nishimura Y, Nakayama J
Mol Cell 47 228-41 (2012)
PMID: 22727667

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158414 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromo domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 75 amino acids
Theoretical weight: 8.91 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q10103 (Residues: 1-75; Coverage: 8%)
Gene names: SPAC18G6.02c, chp1
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Histone H3 Chain: B
Molecule details ›
Chain: B
Length: 18 amino acids
Theoretical weight: 2.03 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided
UniProt:
  • Canonical: P61830 (Residues: 2-18; Coverage: 13%)
Gene names: HHT1, HHT2, N2749, SIN2, YBR010W, YBR0201, YNL031C

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 86%
Refinement method: torsion angle dynamics, simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Post-transcriptional control of gene expression by AUF1: mechanisms, physiological targets, and regulation.
White et al. (2013)
14 more

3 mentions without citation

Beyond the histone tale: HP1α deregulation in breast cancer epigenetics.
Vad-Nielsen et al. (2015)
2 more