› 2uv3

X-ray diffraction
1.8Å resolution

Structure of the signal-regulatory protein (SIRP) alpha domain that binds CD47.

Released:
DOI: 10.2210/pdb2uv3/pdb
PDB entry 2uv3 coloured by chain, front view.
PDB entry 2uv3 coloured by chain, side view.
PDB entry 2uv3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the macrophage signal regulatory protein alpha (SIRPalpha) inhibitory receptor reveals a binding face reminiscent of that used by T cell receptors.
Hatherley D, Harlos K, Dunlop DC, Stuart DI, Barclay AN
J. Biol. Chem. 282 14567-75 (2007)
PMID: 17369261

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160242 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.92 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P78324 (Residues: 31-149; Coverage: 25%)
Gene names: BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, SIRPA
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand MES 2 x MES
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 76.831Å b: 56.266Å c: 82.245Å
α: 90° β: 114.9° γ: 90°
R-values:
R R work R free
0.207 0.204 0.249
Expression system: Cricetulus griseus

Quick links

Citations

16 review citations

Functions and molecular mechanisms of the CD47-SIRPalpha signalling pathway.
Matozaki et al. (2009)
15 more

6 mentions without citation

CD47 update: a multifaceted actor in the tumour microenvironment of potential therapeutic interest.
Sick et al. (2012)
5 more