› 2uwe

X-ray diffraction
2.4Å resolution

Large CDR3a loop alteration as a function of MHC mutation

Released:
DOI: 10.2210/pdb2uwe/pdb
PDB entry 2uwe coloured by chain, front view.
PDB entry 2uwe coloured by chain, side view.
PDB entry 2uwe coloured by chain, top view.
Source organisms:
Primary publication:
Single MHC mutation eliminates enthalpy associated with T cell receptor binding.
Miller PJ, Pazy Y, Conti B, Riddle D, Appella E, Collins EJ
J Mol Biol 373 315-27 (2007)
PMID: 17825839

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-208994 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 275 amino acids
Theoretical weight: 31.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, I
Molecule details ›
Chains: B, I
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Endoplasmic reticulum membrane protein complex subunit 7 Chains: C, J
Molecule details ›
Chains: C, J
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NPA0 (Residues: 4-12; Coverage: 0%)
Gene names: C11orf3, C15orf24, EMC7, HT022, UNQ905/PRO1926
AHIII TCR ALPHA CHAIN Chains: E, L
Molecule details ›
Chains: E, L
Length: 194 amino acids
Theoretical weight: 21.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
T-cell receptor beta chain V region C5 Chains: F, M
Molecule details ›
Chains: F, M
Length: 238 amino acids
Theoretical weight: 26.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04213 (Residues: 8-122; Coverage: 98%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 93.489Å b: 84.178Å c: 121.773Å
α: 90° β: 92.05° γ: 90°
R-values:
R R work R free
0.243 0.24 0.29
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

9 review citations

Conformational changes and flexibility in T-cell receptor recognition of peptide-MHC complexes.
Armstrong et al. (2008)
8 more

6 mentions without citation

Single MHC mutation eliminates enthalpy associated with T cell receptor binding.
Miller et al. (2007)
5 more