› 2uwn

X-ray diffraction
2.35Å resolution

Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand.

Released:
DOI: 10.2210/pdb2uwn/pdb
PDB entry 2uwn coloured by chain, front view.
PDB entry 2uwn coloured by chain, side view.
PDB entry 2uwn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for complement factor H linked age-related macular degeneration.
Prosser BE, Johnson S, Roversi P, Herbert AP, Blaum BS, Tyrrell J, Jowitt TA, Clark SJ, Tarelli E, Uhrín D, Barlow PN, Sim RB, Day AJ, Lea SM
J Exp Med 204 2277-83 (2007)
PMID: 17893204

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140213 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Complement factor H Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 21.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08603 (Residues: 322-506; Coverage: 15%)
Gene names: CFH, HF, HF1, HF2
Sequence domains: Sushi repeat (SCR repeat)
Structure domains: Complement Module, domain 1

Ligands and Environments

Carbohydrate polymer : NEW Components: GU4, YYJ
Carbohydrate polymer
3 bound ligands:
Ligand ACT 1 x ACT
Ligand CL 2 x CL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 74.756Å b: 92.491Å c: 57.311Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

Complement System Part I - Molecular Mechanisms of Activation and Regulation.
Merle et al. (2015)
38 more

9 mentions without citation

Structural basis for complement factor H linked age-related macular degeneration.
Prosser et al. (2007)
8 more