› 2uz9

X-ray diffraction
2.3Å resolution

Human guanine deaminase (guaD) in complex with zinc and its product Xanthine.

Released:
DOI: 10.2210/pdb2uz9/pdb
PDB entry 2uz9 coloured by chain, front view.
PDB entry 2uz9 coloured by chain, side view.
PDB entry 2uz9 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Moche M, Welin M, Arrowsmith C, Berglund H, Busam R, Collins R, Dahlgren LG, Edwards A, Flodin S, Flores A, Graslund S, Hammarstrom M, Hallberg BM, Holmberg-Schiavone L, Johansson I, Kallas A, Karlberg T, Kotenyova T, Lehtio L, Nyman T, Ogg D, Persson C, Sagemark J, Stenmark P, Sundstrom M, Thorsell AG, van den berg S, Weigelt J, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
Guanine + H(2)O = xanthine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine deaminase Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 53.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y2T3 (Residues: 1-454; Coverage: 100%)
Gene names: GDA, KIAA1258
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand XAN 1 x XAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 91.735Å b: 85.713Å c: 78.96Å
α: 90° β: 116.49° γ: 90°
R-values:
R R work R free
0.172 0.169 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links