› 2uzk

X-ray diffraction
2.7Å resolution

Crystal structure of the human FOXO3a-DBD bound to DNA

Released:
DOI: 10.2210/pdb2uzk/pdb
PDB entry 2uzk coloured by chain, front view.
PDB entry 2uzk coloured by chain, side view.
PDB entry 2uzk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human FOXO3a-DBD/DNA complex suggests the effects of post-translational modification.
Tsai KL, Sun YJ, Huang CY, Yang JY, Hung MC, Hsiao CD
Nucleic Acids Res 35 6984-94 (2007)
PMID: 17940099

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115858 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein O3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 97 amino acids
Theoretical weight: 11.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O43524 (Residues: 158-253; Coverage: 14%)
Gene names: FKHRL1, FOXO3, FOXO3A
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*CP*TP*AP*TP*GP*TP*AP*AP*AP*CP*AP*AP*C)-3' Chains: B, D
Molecule details ›
Chains: B, D
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*GP*TP*TP*GP*TP*TP*TP*AP*CP*AP*TP*AP*G)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P61
Unit cell:
a: 41.964Å b: 41.964Å c: 354.817Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.242 0.266
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

36 review citations

The FoxO code.
Calnan et al. (2008)
35 more

17 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
16 more