› 2v01

X-ray diffraction
2.15Å resolution

Recombinant vertebrate calmodulin complexed with Pb

Released:
DOI: 10.2210/pdb2v01/pdb
PDB entry 2v01 coloured by chain, front view.
PDB entry 2v01 coloured by chain, side view.
PDB entry 2v01 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A structural insight into lead neurotoxicity and calmodulin activation by heavy metals.
Kursula P, Majava V
Acta Crystallogr Sect F Struct Biol Cryst Commun 63 653-6 (2007)
PMID: 17671360

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

2 bound ligands:
Ligand PB 8 x PB
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P1
Unit cell:
a: 24.58Å b: 29.95Å c: 53.61Å
α: 88.57° β: 87.03° γ: 82.95°
R-values:
R R work R free
0.191 0.188 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Aluminium and lead: molecular mechanisms of brain toxicity.
Verstraeten et al. (2008)
5 more

5 mentions without citation

Defining potential roles of Pb(2+) in neurotoxicity from a calciomics approach.
Gorkhali et al. (2016)
4 more