› 2v5q

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN

Released:
DOI: 10.2210/pdb2v5q/pdb
PDB entry 2v5q coloured by chain, front view.
PDB entry 2v5q coloured by chain, side view.
PDB entry 2v5q coloured by chain, top view.
Source organisms:
Primary publication:
Structure of wild-type Plk-1 kinase domain in complex with a selective DARPin.
Bandeiras TM, Hillig RC, Matias PM, Eberspaecher U, Fanghänel J, Thomaz M, Miranda S, Crusius K, Pütter V, Amstutz P, Gulotti-Georgieva M, Binz HK, Holz C, Schmitz AA, Lang C, Donner P, Egner U, Carrondo MA, Müller-Tiemann B
Acta Crystallogr D Biol Crystallogr 64 339-53 (2008)
PMID: 18391401

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156755 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 35.97 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53350 (Residues: 32-345; Coverage: 52%)
Gene names: PLK, PLK1
Sequence domains: Protein kinase domain
Structure domains:
DESIGN ANKYRIN REPEAT PROTEIN Chains: C, D
Molecule details ›
Chains: C, D
Length: 167 amino acids
Theoretical weight: 17.84 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 62.329Å b: 135.225Å c: 136.824Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.224
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

11 review citations

Multifaceted polo-like kinases: drug targets and antitargets for cancer therapy.
Strebhardt K. (2010)
10 more

5 mentions without citation

Structural insights for engineering binding proteins based on non-antibody scaffolds.
Gilbreth et al. (2012)
4 more