› 2v64

X-ray diffraction
2.9Å resolution

Crystallographic structure of the conformational dimer of the Spindle Assembly Checkpoint protein Mad2.

Released:
DOI: 10.2210/pdb2v64/pdb
PDB entry 2v64 coloured by chain, front view.
PDB entry 2v64 coloured by chain, side view.
PDB entry 2v64 coloured by chain, top view.
Source organisms:
Primary publication:
The Mad2 conformational dimer: structure and implications for the spindle assembly checkpoint.
Mapelli M, Massimiliano L, Santaguida S, Musacchio A
Cell 131 730-43 (2007)
PMID: 18022367

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163263 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Mitotic spindle assembly checkpoint protein MAD2A Chains: A, C, F
Molecule details ›
Chains: A, C, F
Length: 213 amino acids
Theoretical weight: 24.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q13257 (Residues: 2-205; Coverage: 100%)
Gene names: MAD2, MAD2L1
Sequence domains: HORMA domain
Structure domains: HORMA domain
MBP1 Chains: B, G, I
Molecule details ›
Chains: B, G, I
Length: 12 amino acids
Theoretical weight: 1.45 KDa
Source organism: synthetic construct
Expression system: Not provided
Mitotic spindle assembly checkpoint protein MAD2A Chains: D, E, H
Molecule details ›
Chains: D, E, H
Length: 207 amino acids
Theoretical weight: 23.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q13257 (Residues: 2-205; Coverage: 97%)
Gene names: MAD2, MAD2L1
Sequence domains: HORMA domain
Structure domains: HORMA domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 112.58Å b: 111.88Å c: 131.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.273
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

39 review citations

Protein dynamism and evolvability.
Tokuriki et al. (2009)
38 more

19 mentions without citation

BUB1 and BUBR1: multifaceted kinases of the cell cycle.
Bolanos-Garcia et al. (2011)
18 more