› 2v66

X-ray diffraction
2.1Å resolution

Crystal Structure of the coiled-coil domain of Ndel1 (a.a. 58 to 169) C

Released:
DOI: 10.2210/pdb2v66/pdb
PDB entry 2v66 coloured by chain, front view.
PDB entry 2v66 coloured by chain, side view.
PDB entry 2v66 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the coiled-coil domain of Ndel1 and the basis of its interaction with Lis1, the causal protein of Miller-Dieker lissencephaly.
Derewenda U, Tarricone C, Choi WC, Cooper DR, Lukasik S, Perrina F, Tripathy A, Kim MH, Cafiso DS, Musacchio A, Derewenda ZS
Structure 15 1467-81 (2007)
PMID: 17997972

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclear distribution protein nudE-like 1 Chains: B, C, D, E
Molecule details ›
Chains: B, C, D, E
Length: 111 amino acids
Theoretical weight: 13.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9GZM8 (Residues: 58-168; Coverage: 32%)
Gene names: EOPA, MITAP1, NDEL1, NUDEL
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand HG 2 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 46.431Å b: 73.221Å c: 69.026Å
α: 90° β: 105.4° γ: 90°
R-values:
R R work R free
0.236 0.233 0.31
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Regulators of the cytoplasmic dynein motor.
Kardon et al. (2009)
7 more

5 mentions without citation

PKA phosphorylation of NDE1 is DISC1/PDE4 dependent and modulates its interaction with LIS1 and NDEL1.
Bradshaw et al. (2011)
4 more