› 2v6x

X-ray diffraction
1.98Å resolution

Stractural insight into the interaction between ESCRT-III and Vps4

Released:
DOI: 10.2210/pdb2v6x/pdb
PDB entry 2v6x coloured by chain, front view.
PDB entry 2v6x coloured by chain, side view.
PDB entry 2v6x coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4.
Obita T, Saksena S, Ghazi-Tabatabai S, Gill DJ, Perisic O, Emr SD, Williams RL
Nature 449 735-9 (2007)
PMID: 17928861

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153124 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vacuolar protein sorting-associated protein 4 Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.82 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52917 (Residues: 1-82; Coverage: 19%)
Gene names: CSC1, DID6, END13, GRD13, P9705.10, VPL4, VPS4, VPT10, YPR173C
Sequence domains: MIT (microtubule interacting and transport) domain
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit
DOA4-independent degradation protein 4 Chain: B
Molecule details ›
Chain: B
Length: 54 amino acids
Theoretical weight: 5.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36108 (Residues: 180-232; Coverage: 23%)
Gene names: CHM2, DID4, GRD7, REN1, VPL2, VPS2, VPT14, YKL002W

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1, ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 78.145Å b: 82.27Å c: 21.474Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

53 review citations

The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins.
Raiborg et al. (2009)
52 more

6 mentions without citation

The ESCRT complexes.
Hurley JH. (2010)
5 more