› 2vcy

X-ray diffraction
2.41Å resolution

Crystal Structure of 2-Enoyl Thioester Reductase of Human FAS II

Released:
DOI: 10.2210/pdb2vcy/pdb
PDB entry 2vcy coloured by chain, front view.
PDB entry 2vcy coloured by chain, side view.
PDB entry 2vcy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural enzymological studies of 2-enoyl thioester reductase of the human mitochondrial FAS II pathway: new insights into its substrate recognition properties.
Chen ZJ, Pudas R, Sharma S, Smart OS, Juffer AH, Hiltunen JK, Wierenga RK, Haapalainen AM
J Mol Biol 379 830-44 (2008)
PMID: 18479707

Function and Biology Details

Reaction catalysed: 
An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 344 amino acids
Theoretical weight: 37.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BV79 (Residues: 31-373; Coverage: 92%)
Gene names: CGI-63, MECR, NBRF1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 11 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P42212
Unit cell:
a: 104.62Å b: 104.62Å c: 146.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Current understanding of fatty acid biosynthesis and the acyl carrier protein.
Chan et al. (2010)
4 more

4 mentions without citation

Divergence of multimodular polyketide synthases revealed by a didomain structure.
Zheng et al. (2012)
3 more