› 2ve7

X-ray diffraction
2.88Å resolution

Crystal structure of a bonsai version of the human Ndc80 complex

Released:
DOI: 10.2210/pdb2ve7/pdb
PDB entry 2ve7 coloured by chain, front view.
PDB entry 2ve7 coloured by chain, side view.
PDB entry 2ve7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex.
Ciferri C, Pasqualato S, Screpanti E, Varetti G, Santaguida S, Dos Reis G, Maiolica A, Polka J, De Luca JG, De Wulf P, Salek M, Rappsilber J, Moores CA, Salmon ED, Musacchio A
Cell 133 427-39 (2008)
PMID: 18455984

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127129 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kinetochore protein NDC80 homolog; Kinetochore protein Spc25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 36.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14777 (Residues: 80-286; Coverage: 32%)
  • Canonical: Q9HBM1 (Residues: 118-224; Coverage: 48%)
Gene names: AD024, HEC, HEC1, KNTC2, NDC80, SPBC25, SPC25
Sequence domains:
Structure domains:
Kinetochore protein Nuf2; Kinetochore protein Spc24 Chains: C, D
Molecule details ›
Chains: C, D
Length: 250 amino acids
Theoretical weight: 28.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BZD4 (Residues: 1-169; Coverage: 36%)
  • Canonical: Q8NBT2 (Residues: 122-197; Coverage: 39%)
Gene names: CDCA1, NUF2, NUF2R, SPBC24, SPC24
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand IPH 2 x IPH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 157.653Å b: 248.965Å c: 58.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

86 review citations

The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis.
Carmena et al. (2012)
85 more

12 mentions without citation

Visualizing kinetochore architecture.
Alushin et al. (2011)
11 more