› 2vfx

X-ray diffraction
1.95Å resolution

Structure of the Symmetric Mad2 Dimer

Released:
DOI: 10.2210/pdb2vfx/pdb
PDB entry 2vfx coloured by chain, front view.
PDB entry 2vfx coloured by chain, side view.
PDB entry 2vfx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimer.
Yang M, Li B, Liu CJ, Tomchick DR, Machius M, Rizo J, Yu H, Luo X
PLoS Biol 6 e50 (2008)
PMID: 18318601

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171619 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitotic spindle assembly checkpoint protein MAD2A Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 206 amino acids
Theoretical weight: 23.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13257 (Residues: 1-205; Coverage: 100%)
Gene names: MAD2, MAD2L1
Sequence domains: HORMA domain
Structure domains: HORMA domain

Ligands and Environments

5 bound ligands:
Ligand MG 8 x MG
Ligand CL 37 x CL
Ligand PE4 3 x PE4
Ligand PE3 6 x PE3
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 109.337Å b: 191.407Å c: 154.307Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.209 0.208 0.247
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Regulation of APC/C activators in mitosis and meiosis.
Pesin et al. (2008)
15 more

6 mentions without citation

BUB1 and BUBR1: multifaceted kinases of the cell cycle.
Bolanos-Garcia et al. (2011)
5 more