› 2vgl

X-ray diffraction
2.6Å resolution

AP2 CLATHRIN ADAPTOR CORE

Released:
DOI: 10.2210/pdb2vgl/pdb
PDB entry 2vgl coloured by chain, front view.
PDB entry 2vgl coloured by chain, side view.
PDB entry 2vgl coloured by chain, top view.
Source organisms:
Primary publication:
Molecular architecture and functional model of the endocytic AP2 complex.
Collins BM, McCoy AJ, Kent HM, Evans PR, Owen DJ
Cell 109 523-35 (2002)
PMID: 12086608

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148381 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 621 amino acids
Theoretical weight: 69.66 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18484 (Residues: 1-271, 272-620; Coverage: 66%)
Gene names: Adtab, Ap2a2
Sequence domains: Adaptin N terminal region
Structure domains: Leucine-rich Repeat Variant
AP-2 complex subunit beta Chain: B
Molecule details ›
Chain: B
Length: 591 amino acids
Theoretical weight: 66.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63010 (Residues: 1-591; Coverage: 63%)
Gene names: ADTB2, AP2B1, CLAPB1
Sequence domains: Adaptin N terminal region
Structure domains: Leucine-rich Repeat Variant
AP-2 complex subunit mu Chain: M
Molecule details ›
Chain: M
Length: 435 amino acids
Theoretical weight: 49.73 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84092 (Residues: 1-435; Coverage: 100%)
Gene name: Ap2m1
Sequence domains:
Structure domains:
AP-2 complex subunit sigma Chain: S
Molecule details ›
Chain: S
Length: 142 amino acids
Theoretical weight: 17.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62743 (Residues: 1-142; Coverage: 100%)
Gene names: Ap17, Ap2s1, Claps2
Sequence domains: Clathrin adaptor complex small chain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand IHP 1 x IHP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3121
Unit cell:
a: 121.746Å b: 121.746Å c: 258.124Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.265 0.261 0.339
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

86 review citations

Regulated portals of entry into the cell.
Conner et al. (2003)
85 more

38 mentions without citation

Molecular structure, function, and dynamics of clathrin-mediated membrane traffic.
Kirchhausen et al. (2014)
37 more