› 2vh5

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF HRAS(G12V) - ANTI-RAS FV (disulfide free mutant) COMPLEX

Released:
DOI: 10.2210/pdb2vh5/pdb
PDB entry 2vh5 coloured by chain, front view.
PDB entry 2vh5 coloured by chain, side view.
PDB entry 2vh5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds.
Tanaka T, Rabbitts TH
J Mol Biol 376 749-57 (2008)
PMID: 18187153

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208343 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTI-RAS FV HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 114 amino acids
Theoretical weight: 12.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
ANTI-RAS FV LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 104 amino acids
Theoretical weight: 11.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
GTPase HRas, N-terminally processed Chain: R
Molecule details ›
Chain: R
Length: 166 amino acids
Theoretical weight: 18.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ZN 3 x ZN
Ligand GTP 1 x GTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 75.863Å b: 85.403Å c: 63.084Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.211 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The potential of intracellular antibodies for therapeutic targeting of protein-misfolding diseases.
Cardinale et al. (2008)
7 more

6 mentions without citation

A structural model of a Ras-Raf signalosome.
Mysore et al. (2021)
5 more