› 2vig

X-ray diffraction
1.9Å resolution

Crystal structure of human short-chain acyl CoA dehydrogenase

Released:
DOI: 10.2210/pdb2vig/pdb
PDB entry 2vig coloured by chain, front view.
PDB entry 2vig coloured by chain, side view.
PDB entry 2vig coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pike ACW, Pantic N, Parizotto E, Gileadi O, Ugochukwu E, von Delft F, Weigelt J, Arrowsmith CH, Edwards A, Oppermann U

Function and Biology Details

Reaction catalysed: 
A short-chain acyl-CoA + electron-transfer flavoprotein = a short-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain specific acyl-CoA dehydrogenase, mitochondrial Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 391 amino acids
Theoretical weight: 42.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16219 (Residues: 29-412; Coverage: 93%)
Gene name: ACADS
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 8 x FAD
2 bound ligands:
Ligand EDO 12 x EDO
Ligand COS 5 x COS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 85.714Å b: 157.62Å c: 260.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.231
Expression system: Escherichia coli BL21(DE3)

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