› 2vj0

X-ray diffraction
1.6Å resolution

Crystal structure of the alpha-adaptin appendage domain, from the AP2 adaptor complex, in complex with an FXDNF peptide from amphiphysin1 and a WVXF peptide from synaptojanin P170

Released:
DOI: 10.2210/pdb2vj0/pdb
PDB entry 2vj0 coloured by chain, front view.
PDB entry 2vj0 coloured by chain, side view.
PDB entry 2vj0 coloured by chain, top view.
Source organisms:
Primary publication:
Solitary and repetitive binding motifs for the AP2 complex alpha-appendage in amphiphysin and other accessory proteins.
Olesen LE, Ford MG, Schmid EM, Vallis Y, Babu MM, Li PH, Mills IG, McMahon HT, Praefcke GJ
J Biol Chem 283 5099-109 (2008)
PMID: 17986441

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-126692 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.05 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P17427 (Residues: 690-938; Coverage: 27%)
Gene names: Adtab, Ap2a2
Sequence domains:
Structure domains:
Synaptojanin-1 Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43426 (Residues: 1477-1488; Coverage: 1%)
Gene names: KIAA0910, SYNJ1
Amphiphysin Chain: Q
Molecule details ›
Chain: Q
Length: 7 amino acids
Theoretical weight: 867 Da
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: O08838 (Residues: 324-330; Coverage: 1%)
Gene names: Amph, Amph1

Ligands and Environments

4 bound ligands:
Ligand BEN 1 x BEN
Ligand DTD 1 x DTD
Ligand SO4 3 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2
Unit cell:
a: 144.508Å b: 66.98Å c: 39.73Å
α: 90° β: 94.92° γ: 90°
R-values:
R R work R free
0.182 0.181 0.208
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

The rules of disorder or why disorder rules.
Gsponer et al. (2009)
1 more

6 mentions without citation

Rosetta FlexPepDock ab-initio: simultaneous folding, docking and refinement of peptides onto their receptors.
Raveh et al. (2011)
5 more