› 2vop

X-ray diffraction
2.8Å resolution

Crystal structure of N-terminal domains of Human La protein complexed with RNA oligomer AUUUU

Released:
DOI: 10.2210/pdb2vop/pdb
PDB entry 2vop coloured by chain, front view.
PDB entry 2vop coloured by chain, side view.
PDB entry 2vop coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis reveals conformational plasticity in the recognition of RNA 3' ends by the human La protein.
Kotik-Kogan O, Valentine ER, Sanfelice D, Conte MR, Curry S
Structure 16 852-62 (2008)
PMID: 18547518

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138685 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Lupus La protein Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 22.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05455 (Residues: 4-194; Coverage: 47%)
Gene name: SSB
Sequence domains:
Structure domains:
5'-R(*AP*UP*UP*UP*UP)-3' Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.51 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C222
Unit cell:
a: 96.07Å b: 116.69Å c: 63.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.261 0.29
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Lessons from the crystallographic analysis of small molecule binding to human serum albumin.
Curry S. (2009)
9 more

8 mentions without citation

xRRM: a new class of RRM found in the telomerase La family protein p65.
Singh et al. (2013)
7 more