› 2vux

X-ray diffraction
2.8Å resolution

Human ribonucleotide reductase, subunit M2 B

Released:
DOI: 10.2210/pdb2vux/pdb
PDB entry 2vux coloured by chain, front view.
PDB entry 2vux coloured by chain, side view.
PDB entry 2vux coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Welin M, Moche M, Andersson J, Arrowsmith CH, Berglund H, Busam RD, Collins R, Dahlgren LG, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Herman MD, Johansson A, Johansson I, Kallas A, Karlberg T, Kotenyova T, Lehtio L, Nilsson ME, Nyman T, Persson C, Sagemark J, Schueler H, Svensson L, Thorsell AG, Tresaugues L, van Den Berg S, Weigelt J, Wikstrom M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase subunit M2 B Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 37.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7LG56 (Residues: 17-322; Coverage: 87%)
Gene names: P53R2, RRM2B
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 68.96Å b: 99.47Å c: 132.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.209 0.258
Expression system: Escherichia coli BL21(DE3)

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