› 2vwe

X-ray diffraction
3.4Å resolution

Crystal Structure of Vascular Endothelial Growth Factor-B in Complex with a Neutralizing Antibody Fab Fragment

Released:
DOI: 10.2210/pdb2vwe/pdb
PDB entry 2vwe coloured by chain, front view.
PDB entry 2vwe coloured by chain, side view.
PDB entry 2vwe coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of vascular endothelial growth factor-B in complex with a neutralising antibody Fab fragment.
Leonard P, Scotney PD, Jabeen T, Iyer S, Fabri LJ, Nash AD, Acharya KR
J Mol Biol 384 1203-17 (2008)
PMID: 18930733

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211284 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Vascular endothelial growth factor B Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 19.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49765 (Residues: 22-136, 137-141, 142-149; Coverage: 69%)
Gene names: VEGFB, VRF
Sequence domains: PDGF/VEGF domain
Structure domains: Cystine-knot cytokines
ANTI-VEGF-B MONOCLONAL ANTIBODY Chains: C, J
Molecule details ›
Chains: C, J
Length: 214 amino acids
Theoretical weight: 23.63 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
ANTI-VEGF-B MONOCLONAL ANTIBODY Chains: E, L
Molecule details ›
Chains: E, L
Length: 219 amino acids
Theoretical weight: 23.49 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P21
Unit cell:
a: 86.2Å b: 94.3Å c: 91.3Å
α: 90° β: 102.3° γ: 90°
R-values:
R R work R free
0.286 0.262 0.31
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Grading the commercial optical biosensor literature-Class of 2008: 'The Mighty Binders'.
Rich et al. (2010)
1 more

8 mentions without citation

Tying the knot: the cystine signature and molecular-recognition processes of the vascular endothelial growth factor family of angiogenic cytokines.
Iyer et al. (2011)
7 more