› 2vxs

X-ray diffraction
2.63Å resolution

Structure of IL-17A in complex with a potent, fully human neutralising antibody

Released:
DOI: 10.2210/pdb2vxs/pdb
PDB entry 2vxs coloured by chain, front view.
PDB entry 2vxs coloured by chain, side view.
PDB entry 2vxs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of IL-17A in complex with a potent, fully human neutralizing antibody.
Gerhardt S, Abbott WM, Hargreaves D, Pauptit RA, Davies RA, Needham MR, Langham C, Barker W, Aziz A, Snow MJ, Dawson S, Welsh F, Wilkinson T, Vaugan T, Beste G, Bishop S, Popovic B, Rees G, Sleeman M, Tuske SJ, Coales SJ, Hamuro Y, Russell C
J Mol Biol 394 905-21 (2009)
PMID: 19835883

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212053 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-17A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 137 amino acids
Theoretical weight: 15.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16552 (Residues: 20-155; Coverage: 100%)
Gene names: CTLA8, IL17, IL17A
Sequence domains: Interleukin-17
Structure domains: Cystine-knot cytokines
FAB FRAGMENT Chains: H, I, J, K
Molecule details ›
Chains: H, I, J, K
Length: 225 amino acids
Theoretical weight: 23.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
FAB FRAGMENT Chains: L, M, N, O
Molecule details ›
Chains: L, M, N, O
Length: 216 amino acids
Theoretical weight: 23.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 98.473Å b: 66.708Å c: 203.79Å
α: 90° β: 91.65° γ: 90°
R-values:
R R work R free
0.215 0.213 0.264
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Functional specialization of interleukin-17 family members.
Iwakura et al. (2011)
12 more

16 mentions without citation

Cross-neutralization of influenza A viruses mediated by a single antibody loop.
Ekiert et al. (2012)
15 more