› 2vxt

X-ray diffraction
1.49Å resolution

Crystal structure of human IL-18 complexed to murine reference antibody 125-2H Fab

Released:
DOI: 10.2210/pdb2vxt/pdb
PDB entry 2vxt coloured by chain, front view.
PDB entry 2vxt coloured by chain, side view.
PDB entry 2vxt coloured by chain, top view.
Source organisms:
Primary publication:
Unusual water-mediated antigenic recognition of the proinflammatory cytokine interleukin-18.
Argiriadi MA, Xiang T, Wu C, Ghayur T, Borhani DW
J Biol Chem 284 24478-89 (2009)
PMID: 19553661

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212017 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
MURINE IGG 125-2H Chain: H
Molecule details ›
Chain: H
Length: 216 amino acids
Theoretical weight: 23.26 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Interleukin-18 Chain: I
Molecule details ›
Chain: I
Length: 157 amino acids
Theoretical weight: 18.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q14116 (Residues: 37-193; Coverage: 81%)
Gene names: IGIF, IL18, IL1F4
Sequence domains: Interleukin-1 / 18
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
MURINE IGG 125-2H Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.63 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P21
Unit cell:
a: 44.345Å b: 62.435Å c: 104.734Å
α: 90° β: 100.39° γ: 90°
R-values:
R R work R free
0.164 0.162 0.196
Expression systems:
  • Mus musculus
  • Escherichia coli BL21

Quick links

Citations

3 review citations

Structural biology of the IL-1 superfamily: key cytokines in the regulation of immune and inflammatory responses.
Krumm et al. (2014)
2 more

18 mentions without citation

Structural Basis of IL-1 Family Cytokine Signaling.
Fields et al. (2019)
17 more