› 2w0u

X-ray diffraction
2.84Å resolution

CRYSTAL STRUCTURE OF HUMAN GLYCOLATE OXIDASE IN COMPLEX WITH THE INHIBITOR 5-[(4-CHLOROPHENYL)SULFANYL]- 1,2,3-THIADIAZOLE-4-CARBOXYLATE.

Released:
DOI: 10.2210/pdb2w0u/pdb
PDB entry 2w0u coloured by chain, front view.
PDB entry 2w0u coloured by chain, side view.
PDB entry 2w0u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human glycolate oxidase in complex with the inhibitor 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole.
Bourhis JM, Vignaud C, Pietrancosta N, Guéritte F, Guénard D, Lederer F, Lindqvist Y
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 1246-53 (2009)
PMID: 20054120

Function and Biology Details

Reactions catalysed: 
(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)
Glyoxylate + H(2)O + O(2) = oxalate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193816 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-Hydroxyacid oxidase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 370 amino acids
Theoretical weight: 40.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UJM8 (Residues: 1-370; Coverage: 100%)
Gene names: GOX1, HAO1
Sequence domains: FMN-dependent dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 4 x FMN
1 bound ligand:
Ligand C7C 4 x C7C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P4212
Unit cell:
a: 138.69Å b: 138.69Å c: 186.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.25
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Photorespiratory glycolate-glyoxylate metabolism.
Dellero et al. (2016)
5 more

2 mentions without citation

Structure of human glycolate oxidase in complex with the inhibitor 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole.
Bourhis et al. (2009)
1 more