› 2w2x

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147254 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ras-related C3 botulinum toxin substrate 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 20.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P15153 (Residues: 2-179; Coverage: 93%)
Gene name: RAC2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 14.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P16885 (Residues: 470-913; Coverage: 9%)
Gene name: PLCG2
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 124 amino acids
Theoretical weight: 14.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P16885 (Residues: 470-913; Coverage: 9%)
Gene name: PLCG2
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

2 bound ligands:
Ligand GSP 2 x GSP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 131.57Å b: 84.459Å c: 74.196Å
α: 90° β: 112.21° γ: 90°
R-values:
R R work R free
0.237 0.234 0.294
Expression system: Spodoptera frugiperda

Quick links

Citations

17 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
16 more

5 mentions without citation

Integrating 3D structural information into systems biology.
Murray et al. (2021)
4 more