› 2w4r

X-ray diffraction
2.6Å resolution

Crystal structure of the regulatory domain of human LGP2

Released:
DOI: 10.2210/pdb2w4r/pdb
PDB entry 2w4r coloured by chain, front view.
PDB entry 2w4r coloured by chain, side view.
PDB entry 2w4r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The regulatory domain of the RIG-I family ATPase LGP2 senses double-stranded RNA.
Pippig DA, Hellmuth JC, Cui S, Kirchhofer A, Lammens K, Lammens A, Schmidt A, Rothenfusser S, Hopfner KP
Nucleic Acids Res 37 2014-25 (2009)
PMID: 19208642

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188392 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DHX58 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 142 amino acids
Theoretical weight: 16.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96C10 (Residues: 537-678; Coverage: 21%)
Gene names: D11LGP2E, DHX58, LGP2
Sequence domains: C-terminal domain of RIG-I
Structure domains: RIG-I-like receptor, C-terminal regulatory domain

Ligands and Environments

2 bound ligands:
Ligand HG 4 x HG
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 63.67Å b: 75.62Å c: 147.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.266
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Immune signaling by RIG-I-like receptors.
Loo et al. (2011)
38 more

3 mentions without citation

Structural insights into RNA recognition and activation of RIG-I-like receptors.
Leung et al. (2012)
2 more