› 2w51

X-ray diffraction
2.8Å resolution

Human mesencephalic astrocyte-derived neurotrophic factor (MANF)

Released:
DOI: 10.2210/pdb2w51/pdb
PDB entry 2w51 coloured by chain, front view.
PDB entry 2w51 coloured by chain, side view.
PDB entry 2w51 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the conserved neurotrophic factors MANF and CDNF explains why they are bifunctional.
Parkash V, Lindholm P, Peränen J, Kalkkinen N, Oksanen E, Saarma M, Leppänen VM, Goldman A
Protein Eng Des Sel 22 233-41 (2009)
PMID: 19258449

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mesencephalic astrocyte-derived neurotrophic factor Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 18.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55145 (Residues: 25-182; Coverage: 100%)
Gene names: ARMET, ARP, MANF
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P61
Unit cell:
a: 96.5Å b: 96.5Å c: 35.05Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.281 0.28 0.305
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Novel CDNF/MANF family of neurotrophic factors.
Lindholm et al. (2010)
22 more

5 mentions without citation

The repertoire of protein-sulfatide interactions reveal distinct modes of sulfatide recognition.
Capelluto DGS. (2022)
4 more