› 2w6b

X-ray diffraction
2.8Å resolution

Crystal Structure of the Trimeric beta-PIX Coiled-Coil Domain

Released:
DOI: 10.2210/pdb2w6b/pdb
PDB entry 2w6b coloured by chain, front view.
PDB entry 2w6b coloured by chain, side view.
PDB entry 2w6b coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structures of dimeric GIT1 and trimeric beta-PIX and implications for GIT-PIX complex assembly.
Schlenker O, Rittinger K
J Mol Biol 386 280-9 (2009)
PMID: 19136011

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor 7 Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.65 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O55043 (Residues: 588-638; Coverage: 8%)
Gene names: Arhgef7, Pak3bp, Pixb
Sequence domains: betaPIX coiled coil
Structure domains: L1 transposable element, trimerization domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P63
Unit cell:
a: 43.12Å b: 43.12Å c: 79.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.279 0.278 0.305
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Expanding functions of GIT Arf GTPase-activating proteins, PIX Rho guanine nucleotide exchange factors and GIT-PIX complexes.
Zhou et al. (2016)
2 more

1 mention without citation

Routine phasing of coiled-coil protein crystal structures with AMPLE.
Thomas et al. (2015)