› 2w6f

X-ray diffraction
6Å resolution

Low resolution structures of bovine mitochondrial F1-ATPase during controlled dehydration: Hydration State 2.

Released:
DOI: 10.2210/pdb2w6f/pdb
PDB entry 2w6f coloured by chain, front view.
PDB entry 2w6f coloured by chain, side view.
PDB entry 2w6f coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Improving diffraction by humidity control: a novel device compatible with X-ray beamlines.
Sanchez-Weatherby J, Bowler MW, Huet J, Gobbo A, Felisaz F, Lavault B, Moya R, Kadlec J, Ravelli RB, Cipriani F
Acta Crystallogr D Biol Crystallogr 65 1237-46 (2009)
PMID: 19966409

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133625 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 553 amino acids
Theoretical weight: 59.8 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P19483 (Residues: 1-553; Coverage: 100%)
Gene names: ATP5A1, ATP5A2, ATP5F1A
Sequence domains:
ATP synthase subunit beta, mitochondrial Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 528 amino acids
Theoretical weight: 56.34 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00829 (Residues: 1-528; Coverage: 100%)
Gene names: ATP5B, ATP5F1B
Sequence domains:
ATP synthase subunit gamma, mitochondrial Chain: G
Molecule details ›
Chain: G
Length: 298 amino acids
Theoretical weight: 33.12 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05631 (Residues: 1-298; Coverage: 100%)
Gene names: ATP5C, ATP5C1, ATP5F1C
Sequence domains: ATP synthase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 108Å b: 139.53Å c: 280.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.345 0.345 0.343

Quick links

Citations

6 review citations

Membrane protein structure determination - the next generation.
Moraes et al. (2014)
5 more

2 mentions without citation

A new MR-SAD algorithm for the automatic building of protein models from low-resolution X-ray data and a poor starting model.
Skubák et al. (2018)
1 more