› 2w97

X-ray diffraction
2.29Å resolution

Crystal Structure of eIF4E Bound to Glycerol and eIF4G1 peptide

Released:
DOI: 10.2210/pdb2w97/pdb
PDB entry 2w97 coloured by chain, front view.
PDB entry 2w97 coloured by chain, side view.
PDB entry 2w97 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallization of eIF4E complexed with eIF4GI peptide and glycerol reveals distinct structural differences around the cap-binding site.
Brown CJ, Verma CS, Walkinshaw MD, Lane DP
Cell Cycle 8 1905-11 (2009)
PMID: 19440045

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139167 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Eukaryotic translation initiation factor 4E Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06730 (Residues: 1-217; Coverage: 100%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4E Chain: B
Molecule details ›
Chain: B
Length: 217 amino acids
Theoretical weight: 25.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06730 (Residues: 1-217; Coverage: 100%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4 gamma 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 14 amino acids
Theoretical weight: 1.85 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q04637 (Residues: 609-622; Coverage: 1%)
Gene names: EIF4F, EIF4G, EIF4G1, EIF4GI

Ligands and Environments

4 bound ligands:
Ligand MGO 1 x MGO
Ligand PGE 1 x PGE
Ligand SO4 4 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 84.106Å b: 38.201Å c: 93.687Å
α: 90° β: 101.51° γ: 90°
R-values:
R R work R free
0.199 0.197 0.239
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

Cap and cap-binding proteins in the control of gene expression.
Topisirovic et al. (2011)
3 more

7 mentions without citation

Reversing chemoresistance by small molecule inhibition of the translation initiation complex eIF4F.
Cencic et al. (2011)
6 more