› 2w99

X-ray diffraction
2.8Å resolution

Crystal Structure of CDK4 in complex with a D-type cyclin

Released:
DOI: 10.2210/pdb2w99/pdb
PDB entry 2w99 coloured by chain, front view.
PDB entry 2w99 coloured by chain, side view.
PDB entry 2w99 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human CDK4 in complex with a D-type cyclin.
Day PJ, Cleasby A, Tickle IJ, O'Reilly M, Coyle JE, Holding FP, McMenamin RL, Yon J, Chopra R, Lengauer C, Jhoti H
Proc Natl Acad Sci U S A 106 4166-70 (2009)
PMID: 19237565

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145929 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
G1/S-specific cyclin-D1 Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 30.99 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24385 (Residues: 1-271; Coverage: 92%)
Gene names: BCL1, CCND1, PRAD1
Sequence domains:
Structure domains: Cyclin-like
Cyclin-dependent kinase 4 Chain: B
Molecule details ›
Chain: B
Length: 306 amino acids
Theoretical weight: 34.54 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11802 (Residues: 1-303; Coverage: 99%)
Gene name: CDK4
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 57.023Å b: 64.684Å c: 188.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.27
Expression system: Spodoptera frugiperda

Quick links

Citations

21 review citations

Cyclin D as a therapeutic target in cancer.
Musgrove et al. (2011)
20 more

9 mentions without citation

A new precision medicine initiative at the dawn of exascale computing.
Nussinov et al. (2021)
8 more