› 2wef

X-ray diffraction
1.8Å resolution

Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1) in complex with AMP, PO4 and Magnesium

Released:
DOI: 10.2210/pdb2wef/pdb
PDB entry 2wef coloured by chain, front view.
PDB entry 2wef coloured by chain, side view.
PDB entry 2wef coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Moche M, Schutz P, Arrowsmith CH, Berglund H, Bountra C, Collins R, Dahlgren LG, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson A, Johansson I, Karlberg T, Kotenyova T, Nilsson ME, Nyman T, Persson C, Sagemark J, Schueler H, Siponen MI, Thorsell AG, Tresaugues L, Van Der Berg S, Weigelt J, Welin M, Wisniewska M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
Adenosine 3',5'-bisphosphate + H(2)O = adenosine 5'-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3'(2'),5'-bisphosphate nucleotidase 1 Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 33.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95861 (Residues: 6-308; Coverage: 98%)
Gene name: BPNT1
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand PO4 1 x PO4
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Unit cell:
a: 38.98Å b: 67.664Å c: 100.394Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.144 0.189
Expression system: Escherichia coli BL21(DE3)

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