› 2wew

X-ray diffraction
1.95Å resolution

Crystal structure of human apoM in complex with myristic acid

Released:
DOI: 10.2210/pdb2wew/pdb
PDB entry 2wew coloured by chain, front view.
PDB entry 2wew coloured by chain, side view.
PDB entry 2wew coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Serendipitous fatty acid binding reveals the structural determinants for ligand recognition in apolipoprotein M.
Sevvana M, Ahnström J, Egerer-Sieber C, Lange HA, Dahlbäck B, Muller YA
J Mol Biol 393 920-36 (2009)
PMID: 19733574

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apolipoprotein M Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 19.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95445 (Residues: 18-188; Coverage: 100%)
Gene names: APOM, G3A, HSPC336, NG20
Sequence domains: ApoM domain
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand MYR 1 x MYR
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 49.94Å b: 49.94Å c: 144.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Sphingosine 1-phosphate in coagulation and inflammation.
Obinata et al. (2012)
16 more

3 mentions without citation

Evidence that immunization with TP0751, a bipartite Treponema pallidum lipoprotein with an intrinsically disordered region and lipocalin fold, fails to protect in the rabbit model of experimental syphilis.
Luthra et al. (2020)
2 more