› 2wfn

X-ray diffraction
3.2Å resolution

Filamin A actin binding domain

Released:
DOI: 10.2210/pdb2wfn/pdb
PDB entry 2wfn coloured by chain, front view.
PDB entry 2wfn coloured by chain, side view.
PDB entry 2wfn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human filamin A actin-binding domain.
Ruskamo S, Ylänne J
Acta Crystallogr D Biol Crystallogr 65 1217-21 (2009)
PMID: 19923718

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Filamin-A Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 31.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21333 (Residues: 1-278; Coverage: 11%)
Gene names: FLN, FLN1, FLNA
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 111.5Å b: 125.9Å c: 128.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.225 0.243
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Filamins in mechanosensing and signaling.
Razinia et al. (2012)
1 more

2 mentions without citation

ASB2α, an E3 ubiquitin ligase specificity subunit, regulates cell spreading and triggers proteasomal degradation of filamins by targeting the filamin calponin homology 1 domain.
Razinia et al. (2013)
1 more