› 2wfr

X-ray diffraction
1.95Å resolution

Crystal structure of the N-terminal signalling domain of human Dhh with calcium

Released:
DOI: 10.2210/pdb2wfr/pdb
PDB entry 2wfr coloured by chain, front view.
PDB entry 2wfr coloured by chain, side view.
PDB entry 2wfr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HHIP.
Bishop B, Aricescu AR, Harlos K, O'Callaghan CA, Jones EY, Siebold C
Nat Struct Mol Biol 16 698-703 (2009)
PMID: 19561611

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Desert hedgehog protein N-product Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 18.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43323 (Residues: 39-195; Coverage: 42%)
Gene name: DHH
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 2 x CA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 40.13Å b: 41.88Å c: 83.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.225
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Mechanisms and functions of Hedgehog signalling across the metazoa.
Ingham et al. (2011)
22 more

3 mentions without citation

Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HHIP.
Bishop et al. (2009)
2 more