› 2wjy

X-ray diffraction
2.5Å resolution

Crystal structure of the complex between human nonsense mediated decay factors UPF1 and UPF2 Orthorhombic form

Released:
DOI: 10.2210/pdb2wjy/pdb
PDB entry 2wjy coloured by chain, front view.
PDB entry 2wjy coloured by chain, side view.
PDB entry 2wjy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2.
Clerici M, Mourão A, Gutsche I, Gehring NH, Hentze MW, Kulozik A, Kadlec J, Sattler M, Cusack S
EMBO J 28 2293-306 (2009)
PMID: 19556969

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulator of nonsense transcripts 1 Chain: A
Molecule details ›
Chain: A
Length: 800 amino acids
Theoretical weight: 89.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92900 (Residues: 115-925; Coverage: 71%)
Gene names: KIAA0221, RENT1, UPF1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 64.91Å b: 129.14Å c: 311.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

SF1 and SF2 helicases: family matters.
Fairman-Williams et al. (2010)
34 more

11 mentions without citation

Delicate structural coordination of the Severe Acute Respiratory Syndrome coronavirus Nsp13 upon ATP hydrolysis.
Jia et al. (2019)
10 more